Methane production from the effluent of bio-hydrogen fermentation process by anaerobic sludge using statistical method

Naphat Phowan


The effects of substrate concentration, CaCl2concentration and initial pH of substrate on methane yield (MY) in methane fermentation from the effluent of biohydrogen production process using cassava pulp hydrolysate by anaerobic seed sludge were investigated in this study. A central composite design (CCD) and response surface methodology (RSM) were applied to determine the optimum conditions for methane production. The experimental results showed that the substrate concentrations significantly (p < 0.05) affected methane yield, while the CaCl2 concentrations and the initial pH of substrate in the range of 200 to 600 mg/l and 5.5 to 8.5 respectively did not significantly (p > 0.05) affect methane yield. The interactive effects of all variables on methane yield were significant (p < 0.05). A maximum methane yield of 1984.47 ml CH4.g-1VSadded was obtained under the optimum conditions, i.e.,  substrate concentration of 10,125 mg COD.L-1, CaCl2 concentration of 553.5 mg L-1 and initial pH of 7.46. Verification experiment of the estimated optimum conditions confirmed that the RSM and CCD were useful tools and relevant for optimizing the methane  production from the effluent of bio-hydrogen fermentation of cassava pulp hydrolysate.


Response surface methodology (RSM); methane production; effluent of bio-hydrogen process; central composite design (CCD); cassava pulp hydrolysate

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